PRODUCTION OF CRUDE AMYLASE FROM BACILLUS SUBTILIS ISOLATED FROM WARM WATER
ABSTRACT
This work investigated the feasibility of generating crude thermostable
amylase from Bacillus subtilis isolated from warm water, the entire work
focused on it activity of the enzyme in terms of temperature, pH and
substrate concentration effect. The aim of the findings was to isolate and
determine the effect of temperature, pH and substrate concentration on
the activity of crude amylase from Bacillus subtilis. Glucose oxidase
method was used to determine the activity of the amylase. At the
production time course, amylase was observed to have a maximum yield
of (8.4 μmol/min) at 96 hours of incubation. Maximum crude amylase
was observed at pH 7.0 and temperature 60oC (2.47μmol/min). The
crude amylase production amylase in this current research might be
suitable for applications in industries that require high temperature and
neutral pH.
Keywords: Crude Amylase, Bacillus Subtilis, Warm Water and
Thermostable
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